PoplarV1, Main, Exploration, bibRecord, 003035

Isolation and characterization of osmotic stress-induced genes in poplar cells by suppression subtractive hybridization and cDNA microarray analysis.

Identifieur interne : 003035 ( Main/Exploration ); précédent : 003034; suivant : 003036

Isolation and characterization of osmotic stress-induced genes in poplar cells by suppression subtractive hybridization and cDNA microarray analysis.

Auteurs : Eun-Kyung Bae [Corée du Sud] ; Hyoshin Lee ; Jae-Soon Lee ; Eun-Woon Noh

Source :

Plant physiology and biochemistry : PPB [ 1873-2690 ]

RBID : pubmed:19962907

Descripteurs français

English descriptors

KwdEn :
- Adaptation, Physiological (genetics), Aquaporins (genetics), Aquaporins (metabolism), Cell Wall (genetics), Cell Wall (metabolism), Cysteine Proteases (genetics), Cysteine Proteases (metabolism), DNA, Complementary (MeSH), Gene Expression Regulation (MeSH), Genes, Plant (MeSH), Mannitol (MeSH), Nucleic Acid Hybridization (methods), Oligonucleotide Array Sequence Analysis (methods), Osmosis (MeSH), Populus (genetics), Signal Transduction (genetics), Stress, Physiological (genetics), Transcription, Genetic (MeSH), Up-Regulation (MeSH).
MESH :
- chemical , genetics : Aquaporins, Cysteine Proteases.
- genetics : Adaptation, Physiological, Cell Wall, Populus, Signal Transduction, Stress, Physiological.
- chemical , metabolism : Aquaporins, Cell Wall, Cysteine Proteases.
- methods : Nucleic Acid Hybridization, Oligonucleotide Array Sequence Analysis.
- chemical : DNA, Complementary, Gene Expression Regulation, Genes, Plant, Mannitol, Osmosis, Transcription, Genetic, Up-Regulation.

Abstract

Osmotic stress induces changes in the expression of various genes including those associated with drought tolerance, cell wall metabolism and defense. We isolated 852 cDNA clones, the expression of which is induced by osmotic stress, from cells of a hybrid poplar (Populus alba x Populus tremula var. glandulosa) by suppression subtractive hybridization after mannitol treatment. We examined how stress affected their expression using cDNA microarray analysis, which identified 104 genes significantly up-regulated by osmotic stress. These include genes with functions related to transcription, signal transduction, cell wall metabolism and defense. Other gene transcripts encoding cysteine protease and aquaporin are also up-regulated during osmotic stress. The function of about one-third of the genes in poplar cells that were significantly up-regulated by stress is not known, suggesting that the cell suspension may offer an opportunity of finding novel genes otherwise never expressed and that we still need more information at the molecular level.

DOI: 10.1016/j.plaphy.2009.11.002
PubMed: 19962907

Affiliations:

Corée du Sud

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Le document en format XML

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<author><name sortKey="Bae, Eun Kyung" sort="Bae, Eun Kyung" uniqKey="Bae E" first="Eun-Kyung" last="Bae">Eun-Kyung Bae</name>
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<author><name sortKey="Lee, Hyoshin" sort="Lee, Hyoshin" uniqKey="Lee H" first="Hyoshin" last="Lee">Hyoshin Lee</name>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adaptation, Physiological (genetics)</term>
<term>Aquaporins (genetics)</term>
<term>Aquaporins (metabolism)</term>
<term>Cell Wall (genetics)</term>
<term>Cell Wall (metabolism)</term>
<term>Cysteine Proteases (genetics)</term>
<term>Cysteine Proteases (metabolism)</term>
<term>DNA, Complementary (MeSH)</term>
<term>Gene Expression Regulation (MeSH)</term>
<term>Genes, Plant (MeSH)</term>
<term>Mannitol (MeSH)</term>
<term>Nucleic Acid Hybridization (methods)</term>
<term>Oligonucleotide Array Sequence Analysis (methods)</term>
<term>Osmosis (MeSH)</term>
<term>Populus (genetics)</term>
<term>Signal Transduction (genetics)</term>
<term>Stress, Physiological (genetics)</term>
<term>Transcription, Genetic (MeSH)</term>
<term>Up-Regulation (MeSH)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>ADN complémentaire (MeSH)</term>
<term>Adaptation physiologique (génétique)</term>
<term>Aquaporines (génétique)</term>
<term>Aquaporines (métabolisme)</term>
<term>Cysteine proteases (génétique)</term>
<term>Cysteine proteases (métabolisme)</term>
<term>Gènes de plante (MeSH)</term>
<term>Hybridation d'acides nucléiques (méthodes)</term>
<term>Mannitol (MeSH)</term>
<term>Osmose (MeSH)</term>
<term>Paroi cellulaire (génétique)</term>
<term>Paroi cellulaire (métabolisme)</term>
<term>Populus (génétique)</term>
<term>Régulation de l'expression des gènes (MeSH)</term>
<term>Régulation positive (MeSH)</term>
<term>Stress physiologique (génétique)</term>
<term>Séquençage par oligonucléotides en batterie (méthodes)</term>
<term>Transcription génétique (MeSH)</term>
<term>Transduction du signal (génétique)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Aquaporins</term>
<term>Cysteine Proteases</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Adaptation, Physiological</term>
<term>Cell Wall</term>
<term>Populus</term>
<term>Signal Transduction</term>
<term>Stress, Physiological</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Adaptation physiologique</term>
<term>Aquaporines</term>
<term>Cysteine proteases</term>
<term>Paroi cellulaire</term>
<term>Populus</term>
<term>Stress physiologique</term>
<term>Transduction du signal</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Aquaporins</term>
<term>Cell Wall</term>
<term>Cysteine Proteases</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Nucleic Acid Hybridization</term>
<term>Oligonucleotide Array Sequence Analysis</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Aquaporines</term>
<term>Cysteine proteases</term>
<term>Paroi cellulaire</term>
</keywords>
<keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Hybridation d'acides nucléiques</term>
<term>Séquençage par oligonucléotides en batterie</term>
</keywords>
<keywords scheme="MESH" type="chemical" xml:lang="en"><term>DNA, Complementary</term>
<term>Gene Expression Regulation</term>
<term>Genes, Plant</term>
<term>Mannitol</term>
<term>Osmosis</term>
<term>Transcription, Genetic</term>
<term>Up-Regulation</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>ADN complémentaire</term>
<term>Gènes de plante</term>
<term>Mannitol</term>
<term>Osmose</term>
<term>Régulation de l'expression des gènes</term>
<term>Régulation positive</term>
<term>Transcription génétique</term>
</keywords>
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<front><div type="abstract" xml:lang="en">Osmotic stress induces changes in the expression of various genes including those associated with drought tolerance, cell wall metabolism and defense. We isolated 852 cDNA clones, the expression of which is induced by osmotic stress, from cells of a hybrid poplar (Populus alba x Populus tremula var. glandulosa) by suppression subtractive hybridization after mannitol treatment. We examined how stress affected their expression using cDNA microarray analysis, which identified 104 genes significantly up-regulated by osmotic stress. These include genes with functions related to transcription, signal transduction, cell wall metabolism and defense. Other gene transcripts encoding cysteine protease and aquaporin are also up-regulated during osmotic stress. The function of about one-third of the genes in poplar cells that were significantly up-regulated by stress is not known, suggesting that the cell suspension may offer an opportunity of finding novel genes otherwise never expressed and that we still need more information at the molecular level.</div>
</front>
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<Month>09</Month>
<Day>01</Day>
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<DateRevised><Year>2020</Year>
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<ArticleTitle>Isolation and characterization of osmotic stress-induced genes in poplar cells by suppression subtractive hybridization and cDNA microarray analysis.</ArticleTitle>
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<Abstract><AbstractText>Osmotic stress induces changes in the expression of various genes including those associated with drought tolerance, cell wall metabolism and defense. We isolated 852 cDNA clones, the expression of which is induced by osmotic stress, from cells of a hybrid poplar (Populus alba x Populus tremula var. glandulosa) by suppression subtractive hybridization after mannitol treatment. We examined how stress affected their expression using cDNA microarray analysis, which identified 104 genes significantly up-regulated by osmotic stress. These include genes with functions related to transcription, signal transduction, cell wall metabolism and defense. Other gene transcripts encoding cysteine protease and aquaporin are also up-regulated during osmotic stress. The function of about one-third of the genes in poplar cells that were significantly up-regulated by stress is not known, suggesting that the cell suspension may offer an opportunity of finding novel genes otherwise never expressed and that we still need more information at the molecular level.</AbstractText>
<CopyrightInformation>Copyright 2009 Elsevier Masson SAS. All rights reserved.</CopyrightInformation>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Bae</LastName>
<ForeName>Eun-Kyung</ForeName>
<Initials>EK</Initials>
<AffiliationInfo><Affiliation>Forest Biotechnology Division, Korea Forest Research Institute, 44-3 Omokchundong, Suwon 441-350, Republic of Korea.</Affiliation>
</AffiliationInfo>
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<Author ValidYN="Y"><LastName>Lee</LastName>
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<Author ValidYN="Y"><LastName>Noh</LastName>
<ForeName>Eun-Woon</ForeName>
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<Language>eng</Language>
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<MedlineJournalInfo><Country>France</Country>
<MedlineTA>Plant Physiol Biochem</MedlineTA>
<NlmUniqueID>9882449</NlmUniqueID>
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Isolation and characterization of osmotic stress-induced genes in poplar cells by suppression subtractive hybridization and cDNA microarray analysis.

Isolation and characterization of osmotic stress-induced genes in poplar cells by suppression subtractive hybridization and cDNA microarray analysis.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki